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- Title
Actin is part of pre-initiation complexes and is necessary for transcription by RNA polymerase II.
- Authors
Hofmann, Wilma A.; Stojiljkovic, Ljuba; Fuchsova, Beata; Vargas, Gabriela M.; Mavrommatis, Evangelos; Philimonenko, Vlada; Kysela, Katarina; Goodrich, James A.; Lessard, James L.; Hope, Thomas J.; Hozak, Pavel; de Lanerolle, Primal
- Abstract
Actin is abundant in the nucleus and has been implicated in transcription; however, the nature of this involvement has not been established. Here we demonstrate thatß-actin is critically involved in transcription because antibodies directed againstß-actin, but not muscle actin, inhibited transcription in vivo and in vitro. Chromatin immunoprecipitation assays demonstrated the recruitment of actin to the promoter region of the interferon-?-inducible MHC2TA gene as well as the interferon-a-inducible G1P3 gene. Further investigation revealed that actin and RNA polymerase II co-localize in vivo and also co-purify. We employed an in vitro system with purified nuclear components to demonstrate that antibodies toß-actin block the initiation of transcription. This assay also demonstrates thatß-actin stimulates transcription by RNA polymerase II. Finally, DNA-binding experiments established the presence ofß-actin in pre-initiation complexes and also showed that the depletion of actin prevented the formation of pre-initiation complexes. Together, these data suggest a fundamental role for actin in the initiation of transcription by RNA polymerase II.
- Subjects
ACTIN; NUCLEIC acids; RNA polymerases; IMMUNOGLOBULINS; ANTINEOPLASTIC agents; LYMPHOKINES
- Publication
Nature Cell Biology, 2004, Vol 6, Issue 11, p1094
- ISSN
1465-7392
- Publication type
Article
- DOI
10.1038/ncb1182