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- Title
PARTIAL CHARACTERIZATION OF CELLULASE ENZYME FROM COW DUNG DUMPING SITE SOIL RESIDENT THERMOSTABLE BACTERIA AND BIOETHANOL PRODUCTION.
- Authors
Thomas, Achamma; M., Laxmi; Benny, Aleena; Jayan, Shilpa
- Abstract
The intensifying apprehensions about scarcity of fossil fuels, emission of greenhouse gasses and air pollution by incomplete combustion of fossils have also resulted in a mounting focus on the use of cellulases to perform enzyme hydrolysis of the lignocellulosic materials for the generation of bioethanol. The aim of this study was to isolate potential thermotolerant cellulase producing bacteria from natural sources, thereby proceeding to purification and characterization of the enzyme. Cow dung dumping soil was found to have innumerable versatile bacteria on primary screening and thus used as a source for obtaining desirable cellulase producers. The obtained isolates were screened for their cellulase activity with carboxy methyl cellulose as substrate. A temperature of 50°C was selected for culturing bacterial isolates for subsequent studies to test for their thermostability. Morphological and molecular identification were performed for identification of strain as Bacillus licheniformis. The enzyme was partially purified by ammonium sulfate precipitation method followed by dialysis. 40-60 % ammonium sulphate precipitation fraction showed maximum enzyme activity (46.75 U/mg). Dialysis performed showed fivefold increase in the specific activity whilst compared to the cellulase enzymes before characterization. The Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis (SDS-PAGE) was performed to determine the molecular weight of enzyme and was found out to be approximate 48KDa.The purified enzyme was accessible for the bioethanol production as well as industrial exploitation. The exploitation of bacteria in the search for improved enzymes or strategies provides a means to upgrade feasibility for lignocellulosic biomass conversion, ultimately providing means to a 'greener' technology.
- Subjects
CELLULASE; SOIL microbiology; ETHANOL as fuel
- Publication
Journal of Advanced Scientific Research, 2020, Vol 11, p280
- ISSN
0976-9595
- Publication type
Article