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- Title
Plasma-derived exosomal miR-4732-5p is a promising noninvasive diagnostic biomarker for epithelial ovarian cancer.
- Authors
Liu, Jingjing; Yoo, Jigeun; Ho, Jung Yoon; Jung, Yuyeon; Lee, Sanha; Hur, Soo Young; Choi, Youn Jin
- Abstract
Background: Exosomal miRNAs regulate gene expression and play important roles in several diseases. We used exosomal miRNA profiling to investigate diagnostic biomarkers of epithelial ovarian cancer (EOC). Methods: In total, 55 individuals were enrolled, comprising healthy (n = 21) and EOC subjects (n = 34). Small mRNA (smRNA) sequencing and real-time PCR (RT-PCR) were performed to identify potential biomarkers. Receiver operating characteristic (ROC) curves were conducted to determine biomarker sensitivity and specificity. Results: Using smRNA sequencing, we identified seven up-regulated (miR-4732-5p, miR-877-5p, miR-574-3p, let-7a-5p, let-7b-5p, let-7c-5p, and let-7f-5p) and two down-regulated miRNAs (miR-1273f and miR-342-3p) in EOC patients when compared with healthy subjects. Of these, miR-4732-5p and miR-1273f were the most up-regulated and down-regulated respectively, therefore they were selected for RT-PCR analysis. Plasma derived exosomal miR-4732-5p had an area under the ROC curve of 0.889, with 85.7% sensitivity and 82.4% specificity in distinguishing EOC patients from healthy subjects (p<0.0001) and could be a potential biomarker for monitoring the EOC progression from early stage to late stage (p = 0.018). Conclusions: Plasma derived exosomal miR-4732-5p may be a promising candidate biomarker for diagnosing EOC.
- Subjects
OVARIAN epithelial cancer; RECEIVER operating characteristic curves; BIOMARKERS; BIOLOGICAL tags
- Publication
Journal of Ovarian Research, 2021, Vol 14, Issue 1, p1
- ISSN
1757-2215
- Publication type
Article
- DOI
10.1186/s13048-021-00814-z