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- Title
Early myeloid-derived suppressor cells (HLA-DR<sup>−</sup>/<sup>low</sup>CD33<sup>+</sup>CD16<sup>−</sup>) expanded by granulocyte colony-stimulating factor prevent acute graft-versus-host disease (GVHD) in humanized mouse and might contribute to lower GVHD in patients post allo-HSCT
- Authors
Wang, Ke; Lv, Meng; Chang, Ying-Jun; Zhao, Xiang-Yu; Zhao, Xiao-Su; Zhang, Yuan-Yuan; Sun, Yu-Qian; Wang, Zhi-Dong; Suo, Pan; Zhou, Yang; Liu, Dan; Zhai, Shu-Zhen; Hong, Yan; Wang, Yu; Zhang, Xiao-Hui; Xu, Lan-Ping; Liu, Kai-Yan; Huang, Xiao-Jun
- Abstract
Introduction: Myeloid-derived suppressor cells (MDSCs) are proposed to control graft-versus-host disease (GVHD) in allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, the definition of human MDSCs has not yet reached consensus, and the mechanism of MDSCs to control GVHD remains unclear. Methods: Immature myeloid cells (HLA-DR−/lowCD33+CD16−) were tested before and after granulocyte colony-stimulating factor (G-CSF) administration in healthy donor and isolated for suppression assays and co-culture with T cells in vitro. Isolated cells were infused in humanized mice for a xenogeneic model of acute GVHD. One hundred allo-HSCT recipients were enrolled prospectively to assess the role of HLA-DR−/lowCD33+CD16− cells in grafts on the occurrence of acute GVHD. Results: In the present study, G-CSF mobilized HLA-DR−/lowCD33+CD16− cells with immunosuppressive properties in donor peripheral blood. These cells contained more interleukin-10+ and transforming growth factor-beta (TGF-β)+ cells after G-CSF administration and inhibited the proliferation of autologous donor T cells in a TGF-β-dependent manner. Meanwhile, these immature myeloid cells promoted regulatory T cell expansion and induced Th2 differentiation. Importantly, these cells prevented acute GVHD in a humanized mouse model. Moreover, clinical cohort results showed that the number of HLA-DR−/lowCD33+CD16− cells in the donor graft was the only independent risk factor inversely correlated with the incidence of grade II–IV acute GVHD in the recipients (HR 0.388, 95% CI 0.158–0.954, p = 0.039). Conclusion: HLA-DR−/lowCD33+CD16− cells represent functional MDSCs that may control acute GVHD in allo-HSCT.
- Subjects
SUPPRESSOR cells
- Publication
Journal of Hematology & Oncology, 2019, Vol 12, Issue 1, pN.PAG
- ISSN
1756-8722
- Publication type
Article
- DOI
10.1186/s13045-019-0710-0