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- Title
LEGEN DRINK FROM FERMENTED NIRA SIWALAN PREVENTED THE INFLAMMATION OF ILEUM IN RATS INFECTED WITH ESCHERICHIA COLI.
- Authors
Permata, Fajar Shodiq; Widodo, Edwin; Puspita, Yanuriya Yala
- Abstract
Pathogen Escherichia coli causes several digestive diseases in animals. Legen drink is a traditional drink from Indonesia. It is originated from fermented Nira Siwalan (Borassus flabellifer L.) that resulted in several compounds as antibacterials such as acid substance and flavonoid. The study aimed to examine the effect of Legen drink to prevent inflammation by E.coli to ileum rats based on histopathology and IL-1ß expression. Fermented Nira Siwalan made by 0.5% (v/v) of Lactobacillus casei 1x10 CFU/mL fermentation. The research used 20 male, 150-200 grams Wistar rats (Rattus norvegicus) divided into five groups. Legen groups consisted of a negative control (no treatments); positive control (infected 1 mL 1x106 CFU/ml of E. coli orally); and three kinds preventive groups used Legen with various volume doses (1.8 mL/rat/day, 2.7 mL/rat/day, 3.6 mL/rat/day orally, respectively). The preventive was from day 1st until days 16th then infected E.coli same concentrations as positive control from days 10th until days 16th. Ileum rats were collected in days 17th then processed for being Hematoxylin-Eosin and IL-1ß immunohistochemistry tissue slide. Histopathological ileum analyzed descriptively. ImmunoRatio ® measured the expression of IL-1ß continued parametric statistical analysis with p<0.05. The result showed that dose of Legen 3.6 ml/rat/day given the best prevention based on normal villi of ileum and reduction significantly (p<0.05) of IL-1ß expression. These results indicated that compound of Legen could prevent infection from E.coli not to damage the ileal villi and induce inflammation. The conclusion was Legen drink from fermented Nira Siwalan should be an antimicrobial candidate.
- Subjects
INDONESIA; ILEITIS; LABORATORY rats; ESCHERICHIA coli; RATTUS norvegicus; RATS; WHEAT bran
- Publication
Veterinary Practitioner, 2020, Vol 21, p391
- ISSN
0972-4036
- Publication type
Article