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- Title
Molecular identification of endophytic fungi from medicinal plant Huperzia serrata based on rDNA ITS analysis.
- Authors
Xu Chen; Yao Qi; Jian Wei; Zheng Zhang; De Wang; Jin Feng; Bing Gan
- Abstract
Fifty-two endophytic fungi strains with different colony morphologies were isolated from stems, leaves and roots of Huperzia serrata (Thunb. ex Murray) Trevis. collected from Bawangling Reserve of Hainan Province in southern China. They were identified mainly based on rDNA ITS sequences and phylogenetic analysis. The results showed that all strains belonged to four classes, i.e. Sordariomycetes (92.31%), Dothideomycetes (3.85%), Pezizomycetes (1.92%) and Agaricomycetes (1.92%). Forty-seven strains were identified at the genus level, including Glomerella ( Colletotrichum), Hypocrea ( Trichoderma), Pleurostoma, Chaetomium, Coniochaeta ( Lecythophora), Daldinia, Xylaria, Hypoxylon, Nodulisporium, Cazia and Phellinus. As to the other five strains, three were identified at the order level and two at the family level, indicating that a great diversity of fungi taxa exists in H. serrata. Most isolated strains belonged to the genus of Glomerella ( Colletotrichum) and Hypoxylon, twenty-one from Glomerella and its anamorph Colletotrichum (42.3% of total isolated strains) and ten from Hypoxylon (19.2% of total isolated strains). Pleurostoma, Chaetomium, Coniochaeta ( Lecythophora), Daldinia, Xylaria, Hypoxylon, Nodulisporium, Cazia and Phellinus were reported as endophytic fungi isolated from H. serrata for the first time.
- Subjects
HAINAN Sheng (China); CHINA; ENDOPHYTIC fungi; RECOMBINANT DNA; PHYLOGENY; CHAETOMIUM; TRICHODERMA; GLOMERELLA; COLLETOTRICHUM; HYPOXYLON
- Publication
World Journal of Microbiology & Biotechnology, 2011, Vol 27, Issue 3, p495
- ISSN
0959-3993
- Publication type
Article
- DOI
10.1007/s11274-010-0480-x