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- Title
The effect of platelet-derived microparticles in stored apheresis platelet concentrates on polymorphonuclear leucocyte respiratory burst.
- Authors
Xie, R. F.; Hu, P.; Li, W.; Ren, Y. N.; Yang, J.; Yang, Y. M.; Wang, Z. Y.; Fan, H. H.
- Abstract
Background and Objectives Platelet-derived microparticles ( PMPs) and other proinflammatory mediators, which are accumulated during the storage process, might induce transfusion adverse events. We hypothesized that the PMP primed polymorphonuclear neutrophil ( PMN) respiratory burst after the transfusion, which could be linked to the transfusion-related acute lung injury ( TRALI). Materials and Methods The PMPs were isolated by centrifugation of the platelet-free plasma from 10 apheresis platelet concentrates (A- PLTs) at 20 000 × g for 1 h. The PMPs were counted by flow cytometric analysis, followed by Western blotting, that were performed on isolated PMPs. The soluble CD40 ligand ( sCD40L, sCD154) was assayed with ELISA. The priming of the formyl-Met-Leu-Phe ( fMLP)-activated PMN respiratory burst was measured with the hydrogen peroxide production. Results The PMP counts increased by 1·7-folds after 3 days of storage. Meanwhile, sCD40L also significantly increased in PMP fraction isolated from the 3-day stored A- PLTs. Furthermore, Western blotting indicated that sCD40L was involved and concentrated in PMP. The PMPs were able to effectively prime the fMLP-activated PMN respiratory burst, which was partly inhibited by CD154 monoclonal antibody or by filtration with 0·1 μ m membrane. Significant relativity was existed between the PMP counts, sCD40L and priming activity during the A- PLT storage. Conclusion The platelet-derived microparticles, which carried the sCD40L, accumulated in the apheresis platelet concentrates during the 5 days of storage. They primed the fMLP-activated PMN respiration burst, which might be relative to TRALI.
- Subjects
PLATELET-derived growth factor; HEMAPHERESIS; BLOOD platelet aggregation; NEUTROPHIL immunology; BLOOD transfusion; FLOW cytometry
- Publication
Vox Sanguinis, 2014, Vol 106, Issue 3, p234
- ISSN
0042-9007
- Publication type
Article
- DOI
10.1111/vox.12092