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- Title
Comparable profiles of the immunoglobulin heavy chain complementarity determining region (CDR)-3 in CD5<sup>+</sup> and CD5<sup>-</sup> human cord blood B lymphocytes.
- Authors
Kiyoi, H.; Naito, K.; Ohno, R.; Naoe, T.
- Abstract
It has been suggested that CD5+ B cells are closely related to the pathogenesis of autoimmune disease or chronic lymphocytic leukaemia, whereas the origin and physiological role of CD5+ B cells remain controversial. To study the molecular differences between CD5+ and CDS- B cells in terms of immunoglobulin gene structure, we sorted both subsets from new-born cord blood and analysed the complementarity determining region (CDR)-3 profiles of the immunoglobulin heavy chain (IgH) gene. The CDR-3 sequences from both CD5+ and CD5- B cells represented the same incidence and length of N-region, and the same usage of D and JH segments. When translated into amino acids, 24, of 32 clones (75%) and 25 of 37 clones (65.8%) from their respective subset were productive, and the composition of the deduced amino acids were similar between both subsets. These data suggest that CD5+ B cells are not a distinct lineage presenting a biased immunoglobulin repertoire in B cells.
- Subjects
IMMUNOGLOBULINS; LYMPHOCYTES; B cells; CORD blood; AMINO acids; AUTOIMMUNE diseases; IMMUNE system; IMMUNOLOGY
- Publication
Immunology, 1995, Vol 85, Issue 2, p236
- ISSN
0019-2805
- Publication type
Article