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- Title
Tumour necrosis factor-α augments the expression of Fc IgE receptor (FcεRII/CD23) on human monocytic cell lines and down-regulates interleukin-4-driven FcεRII expression on monocytes.
- Authors
Gessl, A.; Willheim, M.; Agis, H.; Spittler, A.; Schedle, A.; Krugluger, W.; Förster, O.; Boltz-Nitulescu, G.
- Abstract
We investigated the expression of the low affinity Fc IgE receptor (FceRII/CD23) on the human monocytic cell lines U937, THP-1, Mono-Mac-6, and cultured human peripheral blood monocytes under stimulation with human tumour necrosis factor-α (TNF-α) and other cytokines. FcεRII was demonstrated by flow cytometry analysis employing the anti-FcεRII monoclonal antibody 3-5. TNF-α alone had a weak but significant stimulating effect on the Fc&epsolon;RII expression on the cell lines U937 and THP-1, and very modestly on Mono-Mac-6 cells. TNF-α strongly synergized with interferon-γ) (IFN-γ) and interleukin-4 (IL-4). IFN-α per se was ineffectual, but was able to increase the TNF-α effect. Furthermore, the action of TNF-α was slightly augmented by human IL-6. Similar effects were noted with TNF-β alone or in combination with other cytokines. Interestingly, on human monocytes TNF-α weakly reduced the basal level of FcεRII, and markedly diminished the IL-4-induced FcεRII expression. Our results indicate that several cytokines may interact in a cytokine network to modulate FcεRII expression on monocytic cell lines. On human blood monocytes, TNF-α, like IFN-γ or IL-6, counteracts the IL-4-induced FcεRII expression. These data suggest different regulatory pathways of FcεRII expression on blood monocytes and myelomonocytic cell lines.
- Subjects
TUMOR necrosis factors; FC receptors; CELL lines; INTERLEUKIN-4; MONOCYTES
- Publication
Immunology, 1993, Vol 78, Issue 3, p476
- ISSN
0019-2805
- Publication type
Article