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- Title
Lactobacillus paracasei subsp. paracasei NTU 101液態發酵培養的 最適氧氣與pH值條件探討.
- Authors
曾子芸; 林姿杏; 潘子明; 林志輝
- Abstract
Despite Lactobacillus paracasei subsp. paracasei NTU 101 (NTU 101) is a well-known multi-functional health food strain, study for its bioreactor production is still absent. Effects of oxygen level and pH on the growth dynamics of NTU 101 were investigated in this study. Results showed that the highest specific growth (0.61 h-1, p < 0.05) rate was achieved by nitrogen sparging and consequently reduced fermentation time to 9 hours (0.56 fold). The optimized fermentation condition for NTU 101 raised 12.89 fold of initial specific productivity. The unit productivity could be significantly increased by 1.53 fold by using alternative media- 10% (w/v) soya milk powder under optimized condition. RT-qPCR analysis revealed that nitrogen sparging down-regulated the expression level of DnaJ, DnaK, sHsp and GroES genes. Introducing of optimized culture pH further reduced expression levels of DnaJ, DnaK and GroES genes, which indicates that the growth of NTU 101 was related to these three genes. The elevated expression levels of Csp, DnaJ, DnaK, sHsp and GroES genes under oxidative, high pH and low pH conditions also suggested that protein misfolding may be the major limit factor to NTU101 growth since these genes were all participated in protein misfold and refold pathways. The relationship between stress pretreatment and survival ability of NTU 101 cells will be investigated with expression level analysis of these genes in the future.
- Publication
Taiwanese Journal of Agricultural Chemistry & Food Science, 2019, Vol 57, Issue 3, p133
- ISSN
1605-2471
- Publication type
Article
- DOI
10.6578/TJACFS.201906_57(3).0003