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- Title
The effect of zebularine on the heat-activated retrotransposon ONSEN in Arabidopsis thaliana and Vigna angularis.
- Authors
Patwira Boonjing; Yukari Masuta; Kosuke Nozawa; Atsushi Kato; Hidetaka Ito
- Abstract
The Ty1/copia-like retrotransposon ONSEN is conserved among Brassica species, as well as in beans, including adzuki bean (Vigna angularis (Willd.) Ohwi & Ohashi), which is one of the economically important crops in Japan. ONSEN has acquired a heat-responsive element that is recognized by plant heat stress defense factors, resulting in its transcription and the production of full-length extrachromosomal DNA under conditions with elevated temperatures. DNA methylation plays an important role in regulating the activation of this transposon in plants. Therefore, chemical inhibition of DNA methyltransferases has been utilized to study the effect of DNA methylation on transposon activation. To understand the effect of DNA methylation on ONSEN activation, Arabidopsis thaliana and adzuki bean seedlings were treated with zebularine, which is known to be an effective chemical demethylation agent. The results showed that ONSEN transcription levels were upregulated in zebularine-treated plants. Extrachromosomal DNA of ONSEN also accumulated in the treated plants.
- Subjects
JAPAN; VIGNA; EXTRACHROMOSOMAL DNA; DNA methylation; HIGH temperatures; BEANS; METHYLTRANSFERASES
- Publication
Genes & Genetic Systems, 2020, Vol 95, Issue 4, p165
- ISSN
1341-7568
- Publication type
Article
- DOI
10.1266/ggs.19-00046