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- Title
Agrobacterium tumefaciens-transient genetic transformation of Habanero pepper (Capsicum chinense Jacq.) leaf explants.
- Authors
Arcos-Ortega, Guadalupe Fabiola; Chan-Kuuk, Rafael Antonio; González-Kantún, Wilma Aracely; Souza-Perera, Ramón; Nakazawa-Ueji, Yumi Elena; Avilés-Berzunza, Elidé; Godoy-Hernández, Gregorio; Lawton, Michael A.; Aguilar, José Juan Zúñiga
- Abstract
Most of the pepper species of the genus Capsicum have been recalcitrant to efficient Agrobacterium tumefaciens-mediated stable or transient, genetic transformation. In the present work, we optimized a protocol for transient transformation of the Habanero pepper (Capsicum chinense Jacq.) through the standardization of several experimental factors. These included the age of the plants, the temperature, the length of co-cultivation, the application of a negative (vacuum) and/or a positive (infiltration) pressure, along with micro injection, the use of acetosyringone during the bacterial culturing, and modification of the pH during the GUS assay to eliminate the endogenous β- glucuronidase activity. The standardized protocol, which yielded nearly 55% fully transformed leaf explants, was used to successfully mobilize two empty binary vectors (pCAMBIA2301 and pCAMex), as well as the C. chinense cDNAs encoding the pathogenesisrelated protein 10 and esterase, respectively.
- Subjects
PLANT genetic transformation; PLANT genetics; LEAF physiology; PLANT proteins; FOLIAR diagnosis; AGROBACTERIUM tumefaciens; HABANERO
- Publication
Electronic Journal of Biotechnology, 2010, Vol 13, Issue 4, p1
- ISSN
0717-3458
- Publication type
Article
- DOI
10.2225/vol13-issue4-fulltext-10