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- Title
Membrane-protein binding measured with solution-phase plasmonic nanocube sensors.
- Authors
Wu, Hung-Jen; Henzie, Joel; Lin, Wan-Chen; Rhodes, Christopher; Li, Zhu; Sartorel, Elodie; Thorner, Jeremy; Yang, Peidong; Groves, Jay T
- Abstract
We describe a solution-phase sensor of lipid-protein binding based on localized surface plasmon resonance (LSPR) of silver nanocubes. When silica-coated nanocubes are mixed in a suspension of lipid vesicles, supported membranes spontaneously assemble on their surfaces. Using a standard laboratory spectrophotometer, we calibrated the LSPR peak shift due to protein binding to the membrane surface and then characterized the lipid-binding specificity of a pleckstrin homology domain protein.
- Subjects
PROTEIN binding; MEMBRANE proteins; DETECTORS; SURFACE plasmon resonance; SILICA; HOMOLOGY (Biochemistry)
- Publication
Nature Methods, 2012, Vol 9, Issue 12, p1189
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.2211