We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Bayesian localization microscopy reveals nanoscale podosome dynamics.
- Authors
Cox, Susan; Rosten, Edward; Monypenny, James; Jovanovic-Talisman, Tijana; Burnette, Dylan T; Lippincott-Schwartz, Jennifer; Jones, Gareth E; Heintzmann, Rainer
- Abstract
We describe a localization microscopy analysis method that is able to extract results in live cells using standard fluorescent proteins and xenon arc lamp illumination. Our Bayesian analysis of the blinking and bleaching (3B analysis) method models the entire dataset simultaneously as being generated by a number of fluorophores that may or may not be emitting light at any given time. The resulting technique allows many overlapping fluorophores in each frame and unifies the analysis of the localization from blinking and bleaching events. By modeling the entire dataset, we were able to use each reappearance of a fluorophore to improve the localization accuracy. The high performance of this technique allowed us to reveal the nanoscale dynamics of podosome formation and dissociation throughout an entire cell with a resolution of 50 nm on a 4-s timescale.
- Subjects
CELLS; MICROSCOPY; LABORATORY techniques; BAYESIAN analysis; XENON lamps; ARC lamps
- Publication
Nature Methods, 2012, Vol 9, Issue 2, p195
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1812