We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cell type–specific gene expression differences in complex tissues.
- Authors
Shen-Orr, Shai S.; Tibshirani, Robert; Khatri, Purvesh; Bodian, Dale L,; Staedtler, Frank; Perry, Nicholas M,; Hastie, Trevor; Sarwal, Minnie M,; Davis, Mark M,; Butte, Atul J.
- Abstract
We describe cell type–specific significance analysis of microarrays (csSAM) for analyzing differential gene expression for each cell type in a biological sample from microarray data and relative cell-type frequencies. First, we validated csSAM with predesigned mixtures and then applied it to whole-blood gene expression datasets from stable post-transplant kidney transplant recipients and those experiencing acute transplant rejection, which revealed hundreds of differentially expressed genes that were otherwise undetectable.
- Subjects
EXPERIMENTAL biology; GENETIC regulation; GENE expression; PROTEIN microarrays; GRAFT rejection
- Publication
Nature Methods, 2010, Vol 7, Issue 4, p287
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1439