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- Title
Immunogenicity and immunoreactivity of Tp0821 recombinant protein from Treponema pallidum.
- Authors
Ning Wu; Ni Li; Liping Hu; Jun He; Junhong Li; Feijun Zhao; Yimou Wu
- Abstract
Treponema pallidum (Tp) is responsible for invading reproductive organs and the skin in early stages, and involves almost all organs/systems at advanced stages. In the present study, screening of the dominant epitope fragment of the Tp outer membrane protein, Tp0821, was performed and the prokaryotic expression vector pQE32/Tp0821 was constructed. The denaturation and dialysis of rTp0821 were achieved through ultrasound, inclusion body washing and dissolution. Experiments in purified rTp0821-immune New Zealand rabbits indicated that the recombinant proteins were of high immunogenicity, and the irritation led a marked humoral immune response in the New Zealand rabbits. Western blot analysis showed that the purified recombinant proteins reacted with the Tp-positive infected serum, confirming the high level of immunoreactivity. The delayed type hypersensitivity of rTp0821 recombinant proteins was positive, indicating that rTp0821 induced a specific cell immune response and was selected as a Tp vaccine candidate protein. The findings of the present study provided novel evidence, which provided information for further investigations on the pathogenic mechanism of Tp and the development of diagnostic reagents.
- Subjects
TREPONEMA pallidum; RECOMBINANT proteins; EPITOPES; MEMBRANE proteins; GENETIC vectors
- Publication
Molecular Medicine Reports, 2017, Vol 16, Issue 1, p851
- ISSN
1791-2997
- Publication type
Article
- DOI
10.3892/mmr.2017.6675