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- Title
Extracellular vesicle isolation and characterization: toward clinical application.
- Authors
Rong Xu; Greening, David W.; Hong-Jian Zhu; Nobuhiro Takahashi; Simpson, Richard J.; Xu, Rong; Zhu, Hong-Jian; Takahashi, Nobuhiro
- Abstract
Two broad categories of extracellular vesicles (EVs), exosomes and shed microvesicles (sMVs), which differ in size distribution as well as protein and RNA profiles, have been described. EVs are known to play key roles in cell-cell communication, acting proximally as well as systemically. This Review discusses the nature of EV subtypes, strategies for isolating EVs from both cell-culture media and body fluids, and procedures for quantifying EVs. We also discuss proteins selectively enriched in exosomes and sMVs that have the potential for use as markers to discriminate between EV subtypes, as well as various applications of EVs in clinical diagnosis.
- Subjects
EXOSOMES; RNA; CELL communication; CELL culture; BODY fluids
- Publication
Journal of Clinical Investigation, 2016, Vol 126, Issue 4, p1152
- ISSN
0021-9738
- Publication type
journal article
- DOI
10.1172/JCI81129