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- Title
Ultrastructural and Functional Characterization of Mitochondrial Dynamics Induced by Human Respiratory Syncytial Virus Infection in HEp-2 Cells.
- Authors
Lara-Hernandez, Ignacio; Muñoz-Escalante, Juan Carlos; Bernal-Silva, Sofía; Noyola, Daniel E.; Wong-Chew, Rosa María; Comas-García, Andreu; Comas-Garcia, Mauricio
- Abstract
Human respiratory syncytial virus (hRSV) is the leading cause of acute lower respiratory tract infections in children under five years of age and older adults worldwide. During hRSV infection, host cells undergo changes in endomembrane organelles, including mitochondria. This organelle is responsible for energy production in the cell and plays an important role in the antiviral response. The present study focuses on characterizing the ultrastructural and functional changes during hRSV infection using thin-section transmission electron microscopy and RT-qPCR. Here we report that hRSV infection alters mitochondrial morphodynamics by regulating the expression of key genes in the antiviral response process, such as Mfn1, VDAC2, and PINK1. Our results suggest that hRSV alters mitochondrial morphology during infection, producing a mitochondrial phenotype with shortened cristae, swollen matrix, and damaged membrane. We also observed that hRSV infection modulates the expression of the aforementioned genes, possibly as an evasion mechanism in the face of cellular antiviral response. Taken together, these results advance our knowledge of the ultrastructural alterations associated with hRSV infection and might guide future therapeutic efforts to develop effective antiviral drugs for hRSV treatment.
- Subjects
RESPIRATORY syncytial virus infections; RESPIRATORY infections in children; MITOCHONDRIA; RESPIRATORY infections; RESPIRATORY syncytial virus
- Publication
Viruses (1999-4915), 2023, Vol 15, Issue 7, p1518
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v15071518