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- Title
Tracing of islet graft survival by way of in vivo fluorescence imaging.
- Authors
Kahraman, Sevim; Dirice, Ercument; Hapil, Fatma Z.; Ertosun, Mustafa G.; Ozturk, Saffet; Griffith, Thomas S.; Sanlioglu, Salih; Sanlioglu, Ahter D.
- Abstract
Background To increase the success rate in xenogeneic islet transplantation, proper assessment of graft mass is required following transplantation. For this reason, we aimed to develop a suitable fluorescence imaging system to monitor islet xenograft survival in diabetic mice. Methods Adenovirus vector encoding enhanced green fluorescent protein-transduced rat pancreatic islets were transplanted under the renal capsule of streptozotocin-induced diabetic mice and the fluorescence signal was quantified over time using a cooled charge-coupled device. Non-fasting blood glucose levels were recorded during the same period. Insulin release from transduced and control islets was detected via enzyme-linked immunosorbent assay. Results Adenovirus vector encoding enhanced green fluorescent protein infection did not alter the function or survival of pancreatic islets post transduction. A direct correlation was found between the number of islets (250-750) transplanted under the kidney capsule and the blood glucose recovery. Conclusions Fluorescence imaging appears to be a useful tool for quantitative assessment of islet cell viability post transplantation and could permit earlier detection of graft rejection. Copyright © 2011 John Wiley & Sons, Ltd.
- Publication
Diabetes/Metabolism Research & Reviews, 2011, Vol 27, Issue 6, p575
- ISSN
1520-7552
- Publication type
Article
- DOI
10.1002/dmrr.1216