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- Title
New Phenolic Lipids from the Leaves of Clausena harmandiana Inhibit SARS-CoV-2 Entry into Host Cells.
- Authors
Chambon, Marion; Herrscher, Charline; Al Halabi, Dana; François, Nathan; Belouzard, Sandrine; Boutet, Stéphanie; Pham, Van Cuong; Doan, Thi Mai Huong; Séron, Karin; Mavingui, Patrick; Litaudon, Marc; El Kalamouni, Chaker; Apel, Cécile
- Abstract
Induced by the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the COVID-19 pandemic underlined the clear need for antivirals against coronaviruses. In an effort to identify new inhibitors of SARS-CoV-2, a screening of 824 extracts prepared from various parts of 400 plant species belonging to the Rutaceae and Annonaceae families was conducted using a cell-based HCoV-229E inhibition assay. Due to its significant activity, the ethyl acetate extract of the leaves of Clausena harmandiana was selected for further chemical and biological investigations. Mass spectrometry-guided fractionation afforded three undescribed phenolic lipids (1–3), whose structures were determined via spectroscopic analysis. The absolute configurations of 1 and 2 were determined by analyzing Mosher ester derivatives. The antiviral activity against SARS-CoV-2 was subsequently shown, with IC50 values of 0.20 and 0.05 µM for 2 and 3, respectively. The mechanism of action was further assessed, showing that both 2 and 3 are inhibitors of coronavirus entry by acting directly on the viral particle. Phenolic lipids from Clausena harmandiana might be a source of new antiviral agents against human coronaviruses.
- Subjects
SARS-CoV-2; ESTER derivatives
- Publication
Molecules, 2023, Vol 28, Issue 14, p5414
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules28145414