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- Title
Heterogeneous set of cell wall teichoic acids in strains of Bacillus subtilis VKM B-760 and VKM B-764.
- Authors
Potekhina, N. V.; Streshinskaya, G. M.; Kozlova, Yu. I.; Kudryashova, E. B.; Senchenkova, S. N.; Shashkov, A. S.; Anan'ina, L. N.
- Abstract
Cell walls of Bacillus subtilis VKM B-760 and VKM B-764 are characterized by heterogeneous composition of teichoic acids. Polymer I with structure -6)-β- D-Gal p-(1→1)- sn-Gro-(3-P-, polymer II with structure -6)-α- D-Glc p-(1→1)- sn-Gro-(3-P-, and a small amount of unsubstituted 1,3-poly(glycerol phosphate) were detected in strain VKM B-760. Strain VKM B-764 contains an analogous set of teichoic acids, but a characteristic feature of polymer II is the presence of disubstituted glycerol residue with α-glucopyranose localization in the integral chain at C-1 hydroxyl and β-glucopyranose as a side branch at C-2 hydroxyl (polymer III): -6)-α- D-Glc p-(1→1)-[β- D-Glc p-(1→2)]- sn-Gro-(3-P-. The structures of polymer I in bacilli and polymer III in Gram-positive bacteria are described for the first time. Teichoic acids were studied by chemical methods and on the basis of combined analysis of one-dimensional 1H-, 13C-, and 31P-NMR spectra, homonuclear two-dimensional 1H/1H COSY, TOCSY, and ROESY, and heteronuclear two-dimensional 1H/13C gHSQC- and HMQC-TOCSY experiments. Simultaneous presence of several different structure teichoic acids in the bacillus cell walls as well as chemotaxonomical perspectives of the application of these polymers as species-specific markers for members of the Bacillus genus is discussed.
- Subjects
BACILLUS subtilis; BACILLUS (Bacteria); GRAM-positive bacteria; GAS chromatography; POLYMERS
- Publication
Biochemistry (00062979), 2009, Vol 74, Issue 12, p1368
- ISSN
0006-2979
- Publication type
Article
- DOI
10.1134/S0006297909120116