We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
OSTEOGENESIS ACTIVITY OF FRACTIONS EXTRACTED FROM Clinacanthus nutans (Burm. F.) Lindau.
- Authors
Pham Thi Thu Phuong; Nguyen Thi Hong Minh; Quach Thi Lien; Nguyen Thi Thanh Huong; Nguyen Thi Mai Phuong
- Abstract
Osteogenesis activity of fractions extracted from Clinacanthus nutans was evaluated on an in vitro model using osteoblast MC3T3-E1 cells. The results showed that all fractions, including ethanol (EtOH), n-hexane, ethyl acetate (EtOAc) and butanol (BuOH) were not significant ly toxic to the osteoblast cells at the test concentrations of 5, 10, 25, and 50 μg/mL. The EtOH and EtOAc fractions exhibited the highest osteogenesis activity in terms of enhancement of alkaline phosphatase (ALP) and mineralization activity of MC3T3-E1 cells, especially the EtOAc fraction which increased ALP activity up to > 30% and mineralization activity up to > 100%. Thus, the EtOAc fraction shows osteogenesis activity through stimulating activites of the two markers for bone generation including ALP and mineralization in osteoblast cells. The fraction is now under extensive investigation to isolate and fully understand the modes of action of the active compounds.
- Subjects
ACANTHACEAE; BONE growth; OSTEOBLASTS; ALKALINE phosphatase; BIOMINERALIZATION
- Publication
Journal of Biology / TẠp chí Sinh HỌc, 2020, Vol 42, Issue 2, p85
- ISSN
0866-7160
- Publication type
Article
- DOI
10.15625/2615-9023/v42n2.14830