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- Title
Elevated Temperature HPLC: Principles and Applications to Small Molecules and Biomolecules.
- Authors
Zhu, Cuiru; Goodall, David M.; Wren, Stephen A. C.
- Abstract
In this article the use of elevated and high temperatures in high performance liquid chromatography (HPLC) is reviewed, encompassing the range ambient to 250 degree Celsius and with examples covering separations of both small molecules and biomolecules. In most instances the objective of using elevated or high temperature is to increase the speed of separation in order to obtain higher efficiencies and faster results, though there are some situations where selectivity can be manipulated through change of temperature. Only recently has the need for rapid analysis of biological macromolecules drawn attention to the use of elevated column temperatures, and in this field, analytical HPLC is routinely performed at temperatures 20-40 degree Celsius above ambient temperature.
- Subjects
HIGH performance liquid chromatography; HIGH temperatures; MOLECULES; BIOMOLECULES; LIQUID chromatography; CHROMATOGRAPHIC analysis
- Publication
LC-GC Europe, 2004, Vol 17, Issue 10, p530
- ISSN
1471-6577
- Publication type
Article