We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Genetic diversity and population structure of advanced clones selected over forty years by a potato breeding program in the USA.
- Authors
Pandey, Jeewan; Scheuring, Douglas C.; Koym, Jeffrey W.; Coombs, Joseph; Novy, Richard G.; Thompson, Asunta L.; Holm, David G.; Douches, David S.; Miller, J. Creighton; Vales, M. Isabel
- Abstract
Knowledge regarding genetic diversity and population structure of breeding materials is essential for crop improvement. The Texas A&M University Potato Breeding Program has a collection of advanced clones selected and maintained in-vitro over a 40-year period. Little is known about its genetic makeup and usefulness for the current breeding program. In this study, 214 potato clones were genotyped with the Infinium Illumina 22 K V3 Potato Array. After filtering, a total of 10,106 single nucleotide polymorphic (SNP) markers were used for analysis. Heterozygosity varied by SNP, with an overall average of 0.59. Three groups of tetraploid clones primarily based on potato market classes, were detected using STRUCTURE software and confirmed by discriminant analysis of principal components. The highest coefficient of differentiation observed between the groups was 0.14. Signatures of selection were uncovered in genes controlling potato flesh and skin color, length of plant cycle and tuberization, and carbohydrate metabolism. A core set of 43 clones was obtained using Core Hunter 3 to develop a sub-collection that retains similar genetic diversity as the whole population, minimize redundancies, and facilitates long-term conservation of genetic resources. The comprehensive molecular characterization of our breeding clone bank collection contributes to understanding the genetic diversity of existing potato resources. This analysis could be applied to other breeding programs and assist in the selection of parents, fingerprinting, protection, and management of the breeding collections.
- Subjects
POTATO genetics; PLANT breeding; CROP improvement; SINGLE nucleotide polymorphisms; CARBOHYDRATE metabolism
- Publication
Scientific Reports, 2021, Vol 11, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-021-87284-x