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- Title
Immunization of rainbow trout, <em>Salmo gairdneri</em> Richardson, against bacterial kidney disease: preliminary efficacy evaluation.
- Authors
McCarthy, D. H.; Croy, T. R.; Amend, D. F.
- Abstract
A bacterin for immunization against bacterial kidney disease of salmonid fishes caused by Rembactcrium salmoninarum is described. Cultures were grown in Evelyn's KDM2 medium containing 10% calf serum in a fermenter under the following conditions: pH 7.2, 15°C, 800 ml/min air, 200 rev/min agitation and 5-15 days of incubation. Possible substitutes for calf serum were 10% horse serum, 0-15% starch and leptospira medium. The bacterins were inactivated with 0-3% formalin and no adjuvants were used. Other tests evaluated pH-lysed bacterin, 50% concentrated bacterin and 50% concentrated pH-lysed bacterin. Juvenile rainbow trout, Satnio gairdneri Richardson, were vaccinated either by intraperitoneal (i.p.) injection, 2 min immersion or 2-step hyperosmotic infiltration. Fish ere held from four to six weeks at 11°C, then challenged by i.p. injection with the homologous virulent bacterium. Fish died from days 19 to 40 after challenge. The best preparation was pH-lysed bacterin given by a single i.p. injection; hyperosmotic and immersion vaccination were not effective. Typically when 80% or more of unvaccinated controls were infected as detected by Gram stain, 10% or less of the vaccinated fish were infected.
- Subjects
IMMUNIZATION; RAINBOW trout; BACTERIAL kidney disease (Fish disease); KIDNEY diseases; BACTERIAL diseases in fishes; SALMONIDAE infections
- Publication
Journal of Fish Diseases, 1984, Vol 7, Issue 1, p65
- ISSN
0140-7775
- Publication type
Article
- DOI
10.1111/j.1365-2761.1984.tb00907.x