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- Title
Phosphatidylcholine-specific phospholipase C but not gamma interferon regulate gene expression and secretion of CC Chemokine Ligand-2 (CCL-2) by human astrocytes during infection byToxoplasma gondii.
- Authors
Durand, François; Brenier-Pinchart, Marie-Pierre; Berger, Francois; Marche, Patrice N.; Grillot, Renée; Pelloux, Hervé
- Abstract
We have used human astrocytoma-derived cells to investigate the cellular responses of central nervous system cells toToxoplasma gondiiinfection. At 24 h post inoculation, the secretion of CCL-2 (or Monocyte Chemotactic Protein-1) was augmented six-fold over the control. This secretion was down-regulated by D609, a specific inhibitor of phosphatidylcholine-dependent phospholipase C (PC-PLC), but not modulated by gamma interferon (IFN-γ). Ribonuclease protection assay analyses showed significant down-regulation of CCL-2 mRNA production during infection byToxoplasma gondiiwhen cells were treated by D609. The mRNA levels of the seven other chemokines studied were not modified by D609. CCL-2 seems to contribute to the cell recruitment during human cerebral reactivation ofToxoplasma gondii. Cellular production of this CC chemokine during toxoplasmosis may be regulated by a PC-PLC-dependent pathway.
- Subjects
TOXOPLASMA gondii; ASTROCYTES; PHOSPHOLIPASE C; LECITHIN; INTERFERONS; CHEMOKINES; IMMUNOLOGY; TOXOPLASMOSIS
- Publication
Parasite Immunology, 2004, Vol 26, Issue 10, p419
- ISSN
0141-9838
- Publication type
Article
- DOI
10.1111/j.0141-9838.2004.00722.x