We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Ligand-mediated retargeting of recombinant adenovirus for gene transfer in vivo.
- Authors
Thoma, C; Wieland, S; Moradpour, D; von Weizsäcker, F; Offensperger, S; Madon, J; Blum, H E; Offensperger, W-B
- Abstract
The development of efficient and safe methods for in vivo gene transfer is central to the success of gene therapy. Recombinant adenoviral vectors, although highly efficient, are limited by the host immune response, potential safety hazards due to obligatory cotransfer of viral proteins, and their broad tissue tropism. Here, we demonstrate in an animal model that host range and tissue tropism of a recombinant adenovirus from a distant species can be modified by complexing adenovirus with a cell-specific ligand. Thus, a replication-deficient lacZ recombinant human adenovirus, which naturally does not infect avian cells, allowed highly efficient and specific gene transfer to the fiver of ducks in vivo when complexed with N-acetylglucosamine, a ligand for the chicken hepatic lectin. This combination of ligand-mediated receptor targeting with adenoviral uptake and intracellular processing of a given gene represents a novel approach to gene therapy of inherited and acquired fiver diseases.
- Subjects
ADENOVIRUS diseases; RECOMBINANT viruses; GENETIC transformation; LIGANDS (Biochemistry)
- Publication
Gene Therapy, 2000, Vol 7, Issue 12, p1039
- ISSN
0969-7128
- Publication type
Article
- DOI
10.1038/sj.gt.3301194