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- Title
Transfection of cultured myoblasts in high serum concentration with DODAC:DOPE liposomes.
- Authors
Vitiello, L; Bockhold, K; Joshi, P B; Worton, R G
- Abstract
The inhibitory effect of serum is one of the main obstacles to the in vivo use of cationic liposomes as a DNA delivery system. We have found that a novel liposome formulation, DODAC:DOPE (1:1) is totally resistant to the inhibitory effects of serum for transfection of cultured myoblasts and myotubes. Transfection with a lacZ reporter gene in the presence of 95% fetal bovine serum gave up to 25% β-gal-positive cells in C2C12 myoblasts and about six-fold less in primary human myoblasts. The lower transgene expression in primary cells does not appear to be a result of less DNA uptake but might result from differences in intracellular trafficking of the complexes. DODAC-based liposomes are unique in their resistance to serum inhibition and may therefore be valuable for the systemic delivery of genetic information to muscle and other tissues.
- Subjects
MYOBLASTS; LIPIDS; LIPOSOMES; SERUM; GENE transfection
- Publication
Gene Therapy, 1998, Vol 5, Issue 10, p1306
- ISSN
0969-7128
- Publication type
Article
- DOI
10.1038/sj.gt.3300729