We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Multiple Tissues Transcriptome of Zig-Zag Eel (Mastacembelus armatus) with Different Growth Rates.
- Authors
Yang, Jinlin; Lu, Baoyue; Yu, Zhide; Zhang, Linan; Chen, Yiman; Chen, Zihui; Han, Chong; Shu, Hu
- Abstract
Simple Summary: In this study, transcriptome sequencing was first performed on the brain, liver, and muscle tissues of 3-month-old M. armatus individuals with different growth rates. A number of growth-related genes and biological pathways that related to skeletal muscle tissue development and insulin-like growth factor binding were screened. Our results suggest that IGFBP-1A, IGFBP-1B, GH, and GHR play an important role in the early developmental stage of M. armatus. These results contribute to providing a reference for further studies on the molecular mechanism of growth and development in teleost fish. In order to explore the main regulatory genes and related pathways of growth traits, transcriptome sequencing was first performed on the brain, liver, and muscle tissues of 3-month-old M. armatus with different growth rates. By comparative transcriptome analysis of fast-growing and slow-growing groups of M. armatus, a total of 2887 DEGs were screened, of which 59 up-regulated genes and 105 down-regulated genes were detected in the brain, 146 up-regulated genes and 202 down-regulated genes were detected in the liver, and 529 up-regulated genes and 1846 down-regulated genes were detected in muscle, including insulin-like growth factor binding protein 1a (IGFBP1A), insulin-like growth factor binding protein 1b (IGFBP1B), myosin, light chain 1 (MYL1), and myoglobin (MB). Through Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, we identified a total of 288 significantly enriched GO entries and 68 significantly enriched KEGG pathways related to growth, such as skeletal muscle tissue development, insulin-like growth factor binding, and the mitotic cell cycle. These key genes and signaling pathways may play a key role in regulating the growth of M. armatus. Digging into the regulatory mechanisms of these key genes will provide a theoretical basis for further exploration of the molecular mechanisms related to the growth and development of M. armatus, and help to breed new varieties of M. armatus with rapid growth.
- Subjects
INSULIN-like growth factor-binding proteins; SKELETAL muscle; SOMATOMEDIN; REGULATOR genes; TRANSCRIPTOMES
- Publication
Animals (2076-2615), 2024, Vol 14, Issue 2, p248
- ISSN
2076-2615
- Publication type
Article
- DOI
10.3390/ani14020248