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- Title
Sequence and Haplotypes Variation of the Ovine Uncoupling Protein-1 Gene (UCP1) and Their Association with Growth and Carcass Traits in New Zealand Romney Lambs.
- Authors
An, Qingming; Zhou, Huitong; Hu, Jiang; Luo, Yuzhu; Hickford, Jon G. H.
- Abstract
Uncoupling protein-1 gene (<italic>UCP1</italic>) plays an important role in the regulation of thermogenesis, energy expenditure, and protection against oxidative stress. In this study, six separate <italic>UCP1</italic> regions: region-1 and region-2 (two parts of the promoter), region-3 and region-4 (two parts of intron 1), region-5 (spanning part of intron 5 and part of exon 6), and region-6 (spanning part of exon 6 and part of the 3′-UTR) from a variety of sheep breeds, were analysed using polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) analyses. In total, 30 single nucleotide polymorphisms (SNPs) were detected. Of these, 14 were located in the promoter, eight were found in intron 1, six were found in intron 5, and one was found in the 3′-UTR. One substitution in exon 6 (c.910A/G) would putatively result in an amino acid change (p.Thr304Ala). Twenty-eight novel SNPs and nine new haplotypes spanning region-2 to region-5 were identified. Of these nine haplotypes, five were common (<italic>B</italic>2<italic>-A</italic>5, <italic>C</italic>2<italic>-A</italic>5, <italic>C</italic>2<italic>-C</italic>5, <italic>A</italic>2<italic>-A</italic>5, and <italic>A</italic>2<italic>-B</italic>5) and four were rare (<italic>C</italic>2<italic>-B</italic>5, <italic>A</italic>2<italic>-C</italic>5, <italic>B</italic>2<italic>-C</italic>5, and <italic>B</italic>2<italic>-B</italic>5) in the sheep investigated. Of the five common haplotypes found in 314 New Zealand Romney sheep for which growth and carcass trait data were available, the presence of <italic>A</italic>2<italic>-B</italic>5 was associated with decreased hot carcass weight (HCW) and loin lean-meat yield (<italic>p</italic> = 0.006, <italic>p</italic> = 0.032, respectively), and the presence of <italic>C</italic>2<italic>-C</italic>5 was associated with a decreased proportion of leg lean-meat yield (<italic>p</italic> = 0.047) in the carcasses. No associations were found with growth traits. These results confirm that ovine <italic>UCP1</italic> is a variable gene and may have value as a genetic marker for sheep breeding.
- Subjects
UNCOUPLING proteins; MITOCHONDRIA; SINGLE nucleotide polymorphisms; GENETIC polymorphisms; EXONS (Genetics)
- Publication
Genes, 2018, Vol 9, Issue 4, p189
- ISSN
2073-4425
- Publication type
Article
- DOI
10.3390/genes9040189