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- Title
Detection of Tn917 Carrying erm(B) Gene in a Clinical Isolates of S. pyogenes.
- Authors
Hassooni, Hanan Raheem; Jasim, Hameed M.; Farhan, Abbas Aboud; Alhusseiny, Adil Hassan
- Abstract
Background: This study was conducted to investigate the genetic organization of erm-carrying Tn917 in clinical isolates of Streptococcus pyogenes. Tn917 is a nonconjugative transposon which is responsible for the spread of erythromycin resistance in bacterial isolates. Over the past two decades, erythromycin resistance rates have increased in S.pyogenes in many countries, To obtain information that may be useful in solving the spread of antimicrobial resistance, antibiotic-resistant genes can be identified as well as their association with mobile genetic elements. Material and Method: A total of 22 isolates of S. pyogenes isolates collected from the infection of upper respiratory system were examined, first by detecting their antibiotic susceptibility against three different antibiotics groups (Macrolides, Tetracycline, and lincosamides) then genomic DNA was extracted from each isolate for detection Tn917 by using specific primers to amplify erm(B) gene carried by this transposable element. Results: Results showed that there is a high level of resistance to erythromycin, (90.9%), then to Minocycline (68.1%), Lincomycin (59%), Tetracycline (54.5%), Clindamycin (50%), Azithromycin and Clarithromycin (36.3%), Doxycycline (31.8%) and then to Oxytetracycline (27.2%). Results also showed that five of S. pyogenes isolates were harboring Tn917 transposable element carrying erm gene. On the other hand, results showed that there are another isolates resistant to erythromycin that may possess a chromosomal or plasmid copy of the erythromycin resistance gene, or may the resistance caused by another structural erythromycin gene carried by other type of transposable elements rather than Tn917. Conclusion: Our findings suggest that the isolates of S. pyogenes are harboring chromosomal copy of Tn917 conferring erythromycin resistance. One possible explanation for the presence of genes at different isolates is due to erm gene, which was most likely located on Tn917.
- Subjects
MOBILE genetic elements; LINCOMYCIN; DRUG resistance in microorganisms; RESPIRATORY infections; MACROLIDE antibiotics; RHINOVIRUSES; STREPTOCOCCUS; FUNGICIDE resistance
- Publication
Indian Journal of Public Health Research & Development, 2019, Vol 10, Issue 10, p2380
- ISSN
0976-0245
- Publication type
Article
- DOI
10.5958/0976-5506.2019.03215.7