We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
New Insight into Secreted Ribonuclease Structure: Binase Is a Natural Dimer.
- Authors
Dudkina, Elena; Kayumov, Airat; Ulyanova, Vera; Ilinskaya, Olga
- Abstract
The biological effects of ribonucleases (RNases), such as the control of the blood vessels growth, the toxicity towards tumour cells and antiviral activity, require a detailed explanation. One of the most intriguing properties of RNases which can contribute to their biological effects is the ability to form dimers, which facilitates efficient RNA hydrolysis and the evasion of ribonuclease inhibitor. Dimeric forms of microbial RNase binase secreted by Bacillus pumilus (former B. intermedius) have only been found in crystals to date. Our study is the first report directly confirming the existence of binase dimers in solution and under natural conditions of enzyme biosynthesis and secretion by bacilli. Using different variants of gel electrophoresis, immunoblotting, size-exclusion chromatography and mass-spectrometry, we revealed that binase is a stable natural dimer with high catalytic activity.
- Subjects
RIBONUCLEASE inhibitor; BLOOD-vessel development; CANCER cells; CELL-mediated cytotoxicity; ANTIVIRAL agents; ENZYMES; CHEMICAL synthesis; CATALYTIC activity
- Publication
PLoS ONE, 2014, Vol 9, Issue 12, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0115818