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- Title
Global analysis of protein structural changes in complex proteomes.
- Authors
Feng, Yuehan; De Franceschi, Giorgia; Kahraman, Abdullah; Soste, Martin; Melnik, Andre; Boersema, Paul J; de Laureto, Patrizia Polverino; Nikolaev, Yaroslav; Oliveira, Ana Paula; Picotti, Paola
- Abstract
Changes in protein conformation can affect protein function, but methods to probe these structural changes on a global scale in cells have been lacking. To enable large-scale analyses of protein conformational changes directly in their biological matrices, we present a method that couples limited proteolysis with a targeted proteomics workflow. Using our method, we assessed the structural features of more than 1,000 yeast proteins simultaneously and detected altered conformations for ∼300 proteins upon a change of nutrients. We find that some branches of carbon metabolism are transcriptionally regulated whereas others are regulated by enzyme conformational changes. We detect structural changes in aggregation-prone proteins and show the functional relevance of one of these proteins to the metabolic switch. This approach enables probing of both subtle and pronounced structural changes of proteins on a large scale.
- Subjects
PROTEIN conformation; GLOBAL analysis (Mathematics); PROTEOMICS; PROTEOLYSIS; CARBON metabolism
- Publication
Nature Biotechnology, 2014, Vol 32, Issue 10, p1036
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/nbt.2999