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- Title
Expression of the serine protease DESC1 correlates directly with normal keratinocyte differentiation and inversely with head and neck squamous cell carcinoma progression.
- Authors
Sedghizadeh, Parish P.; Mallery, Susan R.; Thompson, Sarah J.; Kresty, Laura; Beck, F. Michael; Parkinson, E. Kenneth; Biancamano, John; Lang, James C.
- Abstract
Background. As part of ongoing studies aimed at identifying the molecular events involved in head and neck squamous cell carcinoma progression, we recently isolated a novel serine protease, DESC1. This study was conducted to further characterize DESC1. Methods. Specimens of normal, dysplastic, and carcinomatous oropharyngeal mucosa (n = 31) were evaluated for DESC1 immunoreactivity using standard streptavidin-biotin immunoperoxidase techniques. Between-lesion stain intensity values were analyzed using multiple Wilcoxon tests. DESC1 expression was also evaluated in cultured human keratinocytes after induction of differentiation by calcium challenge, with subsequent real-time reverse transcriptase-polymerase chain reaction quantification. Results. DESC1 immunoreactivity decreased as lesions approached a malignant phenotype. Post hoc testing comparing the different lesion types and DESC1 staining values showed significance between “normal” and “carcinoma” (p = .0017) groups. Induction of normal keratinocyte differentiation by calcium challenge was accompanied by an increase in DESC1 expression (p = .002). Conclusions. These results suggest downregulation of DESC1 during squamous cell carcinoma progression and upregulation during normal epithelial differentiation. © 2005 Wiley Periodicals, Inc. Head Neck28: 432–440, 2006
- Subjects
SQUAMOUS cell carcinoma; SERINE proteinases; IMMUNOHISTOCHEMISTRY; BACTERIAL proteins; POLYMERASE chain reaction; GENETIC regulation
- Publication
Head & Neck, 2006, Vol 28, Issue 5, p432
- ISSN
1043-3074
- Publication type
Article
- DOI
10.1002/hed.20346