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- Title
Enalapril decreases nuclear factor <em>k</em>B activation in the kidney with ureteral obstruction.
- Authors
Morrissey, Jeremiah J.; Klahr, Saulo
- Abstract
The transcription factor nuclear factor kappa B (NF-κB) controls a number of genes associated with tissue inflammation and has been shown to be activated in the kidney with ureteral obstruction. In this investigation, we further explored NF-κB activation in the kidney cortex of rats with unilateral ureteral obstruction. Electrophoretic mobility shift assays combined with antibody supershift/depletion demonstrated that NF-κB subunits p50, p52, c-rel, p65 (ReIA) and ReIB were all activated. lmmunocytochemical analysis using an antibody to the p50 subunit demonstrated activation occurring predominantly in nuclei of tubular cells. Treatment of animals with unilateral ureteral obstruction with an oral ACE inhibitor significantly decreased NF-κB activation. This suggests that the antiinflammatory effect of ACE inhibitors in renal disease is in part due to a blunting of NF-κB activation.
- Subjects
URETERIC obstruction; KIDNEY glomerulus; PROTEINS; INFLAMMATION; GENES; HEREDITY
- Publication
Kidney International, 1997, Vol 52, Issue 4, p926
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1997.414