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- Title
Morphology and Phylogenetic Analyses of Three Novel <italic>Naegleria</italic> Isolated from Freshwaters on Jeju Island, Korea, During the Winter Period.
- Authors
Khwon, Woo Jun; Park, Jong Soo
- Abstract
Abstract: The genus <italic>Naegleria</italic> is one of the best known heterolobosean groups, and is the causative agent of primary amoebic meningoencephalitis. This group is rarely studied in temperate regions during winter. Here, three novel <italic>Naegleria</italic> were isolated from freshwaters on Jeju Island, Korea, during winter. Two isolates were amoeboflagellates, and one of the three amoebae did not undergo enflagellation. All amoebae had eruptive pseudopodia, and the layer of refractile granules around a large nucleus. They formed a cyst with ~2 pores in the cyst stage. The amoeboflagellate form had two flagella and no division in the flagellate stage, and no cytostome. These features are very similar to typical <italic>Naegleria</italic>. Furthermore, our isolates were able to grow at > 30 °C, suggesting that they had different thermophilicity from <italic>Naegleria</italic> in polar regions. All amoebae were largely encysted at 5 or 10 °C, indicating that they were likely encysted during winter. Based on the 18S rRNA gene and the ITS1‐5.8S rRNA gene‐ITS2 sequences, the phylogenetic analyses consistently revealed that the isolates are members of the <italic>Naegleria</italic> group. However, the isolates differ from other species in both phylogenetic trees. Thus, <italic>Naegleria</italic> in cold habitats appeared to have a high degree of novelty, but their thermophilicity may be dependent on locality.
- Subjects
NAEGLERIA; MENINGOENCEPHALITIS; CYSTS (Pathology); MICROBIAL cysts; AMOEBA; RIBOSOMAL RNA genetics
- Publication
Journal of Eukaryotic Microbiology, 2018, Vol 65, Issue 1, p61
- ISSN
1066-5234
- Publication type
Article
- DOI
10.1111/jeu.12434