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- Title
Effect of ScLL and 15d-PGJ 2 on viability and cytokine release in LPS-stimulated fibroblasts: an in vitro study.
- Authors
REIS, Manuella Verdinelli de Paula; de SOUZA, Gabriela Leite; SOARES, Priscilla Barbosa Ferreira; de SOUZA, Maria Aparecida; SOARES, Carlos José; MOURA, Camilla Christian Gomes
- Abstract
This study evaluated the effect of a cyclopentenone-type PG, 15-Deoxy-12,14-PG J2 (15d-PGJ2), and lectin (ScLL) on the viability of human gingival fibroblasts (HGFs), and on IL-6 and TGFß-1 release by these fibroblasts, stimulated with lipopolysaccharide (LPS). HGFs were stimulated with LPS 10 µg/ml and treated with 15d-PGJ2 1 and 2 µg/ml, and ScLL 2 and 5 µg/ml, for 1 and 3h, and then evaluated for viability by MTT assay. Supernatant was collected to detect IL-6 and TGFß-1 release, by ELISA. Positive control was cells kept in Dulbecco's Modified Eagle's Medium, and negative control was those kept in LPS. Data were analyzed by ANOVA and Dunnett's test (α = 0.05). No significant difference was found in viability among experimental groups at 1h (p > 0.05). Percentage of ScLL 5 µg/ml viable cells was similar to that of positive control at evaluated periods (p > 0.05), whereas the other groups had lower levels than the positive control (p < 0.05). IL-6 release was statistically higher for ScLL 5 µg/ml and 15d-PGJ2 2 µg/ml at 1h, compared with the other treated groups and positive control (p < 0.05). No significant differences were found among the groups at 3h (p > 0.05), except for ScLL 2 µg/ml and 15d-PGJ2 1 µg/ml, which showed lower IL-6 release compared with that of negative control (p < 0.05). No significant difference was found among the groups for TGFβ-1 release (p > 0.05). Results indicated that ScLL 5 µg/ml did not interfere in viability, and ScLL 2 µg/ml and 15d-PGJ2 1 µg/ml demonstrated reduced IL-6 release. Tested substances had no effect on TGFβ-1 release.
- Subjects
CYTOKINES; FIBROBLASTS; IN vitro studies; INTERLEUKIN-6; CELL survival
- Publication
Brazilian Oral Research, 2020, Vol 34, p1
- ISSN
1806-8324
- Publication type
Article
- DOI
10.1590/1807-3107bor-2020.vol34.0013