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- Title
Construction of Graphene@Ag-MLF composite structure SERS platform and its differentiating performance for different foodborne bacterial spores.
- Authors
Zhu, Yaodi; Tian, Jiaqi; Li, Miaoyun; Zhao, Lijun; Shi, Jiyong; Liu, Weijia; Liu, Shijie; Liang, Dong; Zhao, Gaiming; Xu, Lina; Yang, Shufeng
- Abstract
A new surface-enhanced Raman spectroscopy (SERS) biosensor of Graphene@Ag-MLF composite structure has been fabricated by loading AgNPs on graphene films. The response of the biosensor is based on plasmonic sensing. The results showed that the enhancement factor of three different spores reached 107 based on the Graphene@Ag-MLF substrate. In addition, the SERS performance was stable, with good reproducibility (RSD<3%). Multivariate statistical analysis and chemometrics were used to distinguish different spores. The accumulated variance contribution rate was up to 96.35% for the top three PCs, while HCA results revealed that the spectra were differentiated completely. Based on optimal principal components, chemometrics of KNN and LS-SVM were applied to construct a model for rapid qualitative identification of different spores, of which the prediction set and training set of LS-SVM achieved 100%. Finally, based on the Graphene@Ag-MLF substrate, the LOD of three different spores was lower than 102 CFU/mL. Hence, this novel Graphene@Ag-MLF SERS substrate sensor was rapid, sensitive, and stable in detecting spores, providing strong technical support for the application of SERS technology in food safety.
- Subjects
BACTERIAL spores; COMPOSITE structures; COMPOSITE construction; SERS spectroscopy; FOOD science; BIOSENSORS
- Publication
Microchimica Acta, 2023, Vol 190, Issue 12, p1
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-023-06031-3