We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Separation of hydrophobically modified hyaluronic acid according to the degree of substitution by gradient elution high performance liquid chromatography.
- Authors
Botha, Carlo; Viktor, Zanelle; Pfukwa, Helen; Pasch, Harald; Moire, Claudine; Farcet, Céline; Brothier, Fabien
- Abstract
Amphiphilic hyaluronic acid (HA), synthesised by modifying HA to varying extents with acrylate groups, was successfully separated according to degree of substitution (DS) using solvent gradient high performance liquid chromatography (HPLC). Two HPLC methods based on the amphiphilic structure of the HA were developed. In the first approach, normal phase gradient HPLC was explored, and separation was based on the interactions of HA’s polar hydroxyl groups with a polar cyano stationary phase. In the second approach, separation was based on the interaction of the hydrophobic acrylate moieties with a non-polar C8 stationary phase (reversed phase gradient HPLC). The separation was optimised by using an electrolyte in the sample solvent to suppress non-covalent interactions and improve the selectivity of the developed method. The photolytic stability of the modified and unmodified HA was also investigated in order to optimise the sample preparation procedure. Furthermore, an alternative method to NMR spectroscopy was developed for determining the DS of HA.ᅟ<graphic></graphic>
- Subjects
GLYCOSAMINOGLYCANS; HYDROPHOBIC interactions; HYALURONIC acid; HIGH performance liquid chromatography; NUCLEAR magnetic resonance spectroscopy
- Publication
Analytical & Bioanalytical Chemistry, 2018, Vol 410, Issue 18, p4259
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-018-1123-6