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- Title
Functional expression of TRAIL and TRAIL-R2 during human megakaryocytic development.
- Authors
Elisabetta Melloni; Paola Secchiero; Claudio Celeghini; Diana Campioni; Vittorio Grill; Lia Guidotti; Giorgio Zauli
- Abstract
The expression and function of surface TRAIL and TRAIL receptors were investigated in primary megakaryocytic cells, generated in serum-free liquid phase from peripheral human CD34+ cells. The surface expression of both TRAIL and death receptor TRAIL-R2 became detectable starting from the early phase of megakaryocytic differentiation (day 6 of culture) and persisted at later (days1014) culture times. On the other hand, death receptor TRAIL-R1, decoy receptors TRAIL-R3, and TRAIL-R4 were barely detectable or undetectable at any time point examined. Addition of recombinant TRAIL at day 6 of culture increased the rate of spontaneous apoptosis of CD34+/CD41dim megakaryoblasts and it significantly decreased the total output of mature megakaryocytic cells evaluated after additional 48 days of culture. Conversely, addition in culture of TRAIL-R2-Fc chimera, which blocked the interaction between endogenous TRAIL and TRAIL-R2 on the surface of cultured megakaryocytic cells, increased the total megakaryocytic cell count. In addition, recombinant TRAIL promoted a small but reproducible increase of maturation in the surviving megakaryocytic cell population, evaluated by both phenotypic analysis and morphology. A similar pro-maturation effect was observed when TRAIL was added to bone marrow-derived CD61+ megakaryocytic cells. Thus, our data suggest a role of TRAIL as a regulator of megakaryocytopoiesis. 2005 Wiley-Liss, Inc.
- Subjects
CELL populations; IMMUNE system; HEMATOPOIETIC system; CELL death
- Publication
Journal of Cellular Physiology, 2005, Vol 204, Issue 3, p975
- ISSN
0021-9541
- Publication type
Article
- DOI
10.1002/jcp.20358