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- Title
Improved insect‐proofing: expressing double‐stranded RNA in chloroplasts.
- Authors
Bally, Julia; Waterhouse, Peter M.; Fishilevich, Elane; Bowling, Andrew J.; Pence, Heather E.; Narva, Kenneth E.
- Abstract
Abstract: RNA interference (RNAi) was discovered almost 20 years ago and has been exploited worldwide to silence genes in plants and animals. A decade later, it was found that transforming plants with an RNAi construct targeting an insect gene could protect the plant against feeding by that insect. Production of double‐stranded RNA (dsRNA) in a plant to affect the viability of a herbivorous animal is termed trans‐kingdom RNAi (TK‐RNAi). Since this pioneering work, there have been many further examples of successful TK‐RNAi, but also reports of failed attempts and unrepeatable experiments. Recently, three laboratories have shown that producing dsRNA in a plant's chloroplast, rather than in its cellular cytoplasm, is a very effective way of delivering TK‐RNAi. Our review examines this potentially game‐changing approach and compares it with other transgenic insect‐proofing schemes. © 2018 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
- Subjects
CHLOROPLASTS; CYTOPLASM; RNA interference; INSECT-resistant packaging; INSECT genes
- Publication
Pest Management Science, 2018, Vol 74, Issue 8, p1751
- ISSN
1526-498X
- Publication type
Article
- DOI
10.1002/ps.4870