We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Reduction of anti-Galα1,3Gal antibodies by infusion of types 2 and 6 gal trisaccharides conjugated to poly- l-lysine.
- Authors
Kuwaki, K.; Knosalla, C.; Moran, K.; Alt, A.; Katopodis, A.G.; Duthaler, R.O.; Schuurman, H.-J.; Awwad, M.; Cooper, D.K.C.
- Abstract
Kuwaki K, Knosalla C, Moran K, Alt A, Katopodis AG, Duthaler RO, Schuurman H-J, Awwad M, Cooper DKC. Reduction of anti-Galα1, 3Gal antibodies by infusion of types 2 and 6 gal trisaccharides conjugated to poly- l-lysine. Xenotransplantation 2004; 11: 210–215. © Blackwell Munksgaard, 2004 To investigate the specificity of anti-Gal α1,3Gal (Gal) antibodies (Abs) with respect to Gal oligosaccharides of types 2 and 6, eight baboons received an intravenous infusion of either a poly- l-lysine conjugate of Gal type 2 (n = 5) or type 6 (n = 3), followed 48 h later by the alternative Gal type 6 or 2 conjugate, respectively. IgM Abs reactive to Gal type 2 were depleted by 80 to 89% by either Gal conjugate. IgM reactive to Gal type 6 was less efficiently depleted by the Gal type 2 conjugate (57% depletion) than the Gal type 6 (82% depletion). Gal-reactive IgG was depleted more slowly and less efficiently by either glycoconjugate (initially by only 28 to 54%). Our results indicate that the Gal type 6 conjugate depletes most anti-Gal IgM, but the Gal type 2 conjugate is less efficient in depleting anti-Gal IgM reactive with type 6. There remain small fractions of antibody that are unadsorbed, particularly of IgG, probably due to their low affinity and distribution in both the intra- and extra-vascular compartments.
- Subjects
IMMUNOGLOBULINS; OLIGOSACCHARIDES; BABOONS; IMMUNOGLOBULIN M; TRANSPLANTATION of organs, tissues, etc.; INTRAVENOUS therapy
- Publication
Xenotransplantation, 2004, Vol 11, Issue 2, p210
- ISSN
0908-665X
- Publication type
Article
- DOI
10.1046/j.1399-3089.2003.00096.x