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- Title
The expression of histamine H<sub>4</sub> receptor mRNA in the skin and other tissues of normal dogs.
- Authors
Eisenschenk, Melissa N. C.; Torres, Sheila M. F.; Oliveira, Simone; Been, Clint S.
- Abstract
The histamine 4 (H4) receptor was first cloned and characterized in 2000 using the human H3 receptor DNA sequence. The H4 receptor has been shown to participate in various aspects of inflammation, such as chemotaxis, upregulation of adhesion molecule expression and modulation of cytokine secretion. The primary goal of this study was to determine whether H4 receptor mRNA is expressed in normal canine skin by performing an RT-PCR. An additional goal was to determine the expression of this receptor in the colon, liver, spleen and kidney. Tissues were collected from five healthy, young-adult pit bull dogs. Samples were immediately placed in RNA later® solution and stored at −20°C until processed. The amplified products in all skin samples in addition to the colon, liver, spleen and kidney (variable expression) had the expected size of 400-500 bp. The sequenced amplicons matched the National Center for Biotechnology Information published sequence for the canine H4 receptor. The study results showed that canine normal skin expresses the H4 receptor mRNA. Further studies using immunohistochemistry should be conducted to demonstrate the expression of the H4 receptor at the protein level and to localize the expression of this receptor in the skin.
- Subjects
HISTAMINE receptors; MESSENGER RNA; NUCLEOTIDE sequence; SKIN inflammation; CHEMOTAXIS; CELL adhesion molecules; CYTOKINES; IMMUNOHISTOCHEMISTRY; THERAPEUTICS
- Publication
Veterinary Dermatology, 2011, Vol 22, Issue 5, p396
- ISSN
0959-4493
- Publication type
Article
- DOI
10.1111/j.1365-3164.2011.00959.x