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- Title
Molecular cloning of a Salmonella typhi LT-like enterotoxin gene.
- Authors
Fernández, M.; Sierra-Madero, J.; de la Vega, H.; Vázquez, M.; López-Vidal, Y.; Ruíz-Palacios, G. M.; Calva, E.
- Abstract
Diarrhoea is a common event during typhoid fever; nevertheless, the possible participation of a diarrhoea-inducing enterotoxin has not been described (Roy et al., 1985). Recombinant bacteriophage λ FDC1 was isolated from a genomic library of Salmonella typhi, the causal agent of typhoid fever, by screening with a probe for the B subunit gene of the heat-labile, cholera-like, Escherichia coli enterotoxin (LT). λ FDC1 codes for an enterotoxin that causes secretion in rat ileal loops, that elongates Chinese hamster ovary (CHO) cells, that is recognized by antibodies against LT, and does not bind in vitro to ganglioside GM1. These results should allow further studies towards elucidating a possible role for the S. typhi enterotoxin in the pathogenesis of typhoid fever.
- Subjects
SALMONELLA typhi; DIARRHEA; ENTEROTOXINS; MOLECULAR cloning; BACTERIAL toxins; MOLECULAR genetics
- Publication
Molecular Microbiology, 1988, Vol 2, Issue 6, p821
- ISSN
0950-382X
- Publication type
Article