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- Title
Culturing human umbilical cord blood: a comparison of mononuclear vs CD34<sup>+</sup> selected cells.
- Authors
Fietz, T; Berdel, W E; Rieder, H; Reufi, B; Hopp, H; Thiel, E; Knauf, W U
- Abstract
We compared UCB mononuclear cells (MNC) with CD34+ selected cells in a serum-free static culture system. Cell number proliferation of MNCs was inferior to CD34+ selected cells. MNCs, however, showed a substantial increase from 0.94% CD34+ cells on day 0 to 5.8% on day 7, whereas in the CD34+selected samples the CD34+ cell content declined continously from 62.2% on day 0 to 27.7% on day 7. The number of CFU-GM increased during culture of both cell fractions. Here, only the MNCs showed a substantial increase in clonogenicity on day 7 and day 14 to 11.1- and 4.1-fold input, respectively. This expansion of the CD34+ progenitor cell pool in the MNCs fraction was at least in part attributable to T cells, since the physical abrogation of T cells blocked this effect. Refeeding and reseeding of cells on day 7 had stimulating effects especially on the CD34+ cells, where cell number proliferation increased from 16.3-fold without to 58.1-fold on day 14. Also, we could find sporadic chromosomal aberrations in four of 100 metaphases examined after 7–20 days of ex vivo expansion. The significance of this observation needs to be clarified in a larger series.
- Subjects
CORD blood; BLOOD cells
- Publication
Bone Marrow Transplantation, 1999, Vol 23, Issue 11, p1109
- ISSN
0268-3369
- Publication type
Article
- DOI
10.1038/sj.bmt.1701776