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- Title
Heat shock protein 70 could enhance porcine epidemic diarrhoea virus replication by interacting with membrane proteins.
- Authors
Park, Jae-Yeon; Ryu, Jihoon; Park, Jung-Eun; Hong, Eui-Ju; Shin, Hyun-Jin
- Abstract
In this study, we investigated the role of heat shock protein 70 (HSP70) in porcine epidemic diarrhoea virus (PEDV) replication. We found that PEDV infection induced strong HSP70 overexpression in the very early stage of infection. We also confirmed that HSP70 overexpression increased the speed of PEDV replication, resulting in the generation of more virions. In contrast, knockout of HSP70 in cells significantly downregulated PEDV protein expression, resulting in a significant reduction in PEDV replication. Most importantly, we confirmed that among the structural proteins of PEDV, membrane (M) proteins have this important role. We found that membrane proteins control cellular HSP70 expression in PEDV-infected cells. We confirmed HSP70/M complex formation by both immunoprecipitation and immunofluorescence assays. Additionally, PEDV M overexpression induced strong HSP70 expression. All our results clearly confirmed that in PEDV-infected cells, the M protein plays a very important role in PEDV replication in collaboration with HSP70.
- Subjects
HEAT shock proteins; MEMBRANE proteins; CYTOSKELETAL proteins; VIRAL replication; DIARRHEA; PORCINE reproductive &; respiratory syndrome
- Publication
Veterinary Research, 2021, Vol 52, Issue 1, p1
- ISSN
0928-4249
- Publication type
Article
- DOI
10.1186/s13567-021-01006-9