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- Title
The effect of pH on cell viability, cell migration, cell proliferation, wound closure, and wound reepithelialization: In vitro and in vivo study.
- Authors
Kruse, Carla R.; Singh, Mansher; Targosinski, Stefan; Sinha, Indranil; Sørensen, Jens A.; Eriksson, Elof; Nuutila, Kristo
- Abstract
Wound microenvironment plays a major role in the process of wound healing. It contains various external and internal factors that participate in wound pathophysiology. The pH is an important factor that influences wound healing by changing throughout the healing process. Several previous studies have investigated the role of pH in relation to pathogens but studies concentrating on the effects of pH on wound healing itself are inconclusive. The purpose of this study was to comprehensively and in a controlled fashion investigate the effect of pH on wound healing by studying its effect on human primary keratinocyte and fibroblast function in vitro and on wound healing in vivo. In vitro, primary human keratinocytes and fibroblasts were cultured in different levels of pH (5.5-12.5) and the effect on cell viability, proliferation, and migration was studied. A rat full-thickness wound model was used to investigate the effect of pH (5.5-9.5) on wound healing in vivo. The effect of pH on inflammation was monitored by measuring IL-1
- Subjects
CELL proliferation; ANIMAL experimentation; BIOLOGICAL models; CELL culture; CELL migration; CELL physiology; FIBROBLASTS; GRANULATION tissue; HYDROGEN-ion concentration; INTERLEUKINS; KERATINOCYTES; MICROBIOLOGICAL assay; RATS; WOUND healing; IN vitro studies; IN vivo studies
- Publication
Wound Repair & Regeneration, 2017, Vol 25, Issue 2, p260
- ISSN
1067-1927
- Publication type
Article
- DOI
10.1111/wrr.12526