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- Title
Peroxidase Mimicking Activity of Palladium Nanocluster Altered by Heparin.
- Authors
Zhang, Qianqian; Zheng, Shanshan; Zhang, Jinli; Li, Wei; Fu, Yan
- Abstract
NADH, composed of metal binding, charged and redox groups, acts as a "three-in-one" template to construct peroxidase mimicking Pd nanoclusters (NCs). The physicochemical properties of Pd NCs are highly dependent upon the molar ratio of [NADH]/[Na2PdCl4], thereby contributing to distinct peroxidase mimicking activities. The ultrasmall Pd nanozyme that contains 30% metallic Pd0 species, exhibits the Km of 0.063 mM toward TMB and 80.8 mM toward H2O2. Notably, addition of heparin enables the enhancement of its peroxidase-like activity in neutral media. A colorimetric assay was well-established at pH 6 for quantitatively monitoring heparin in aqueous solution and biological fluid. The linear response lies in the range of 0.5–25 µg mL−1, with the limit of detection of 1.1 ng mL−1. This work paves a promising pathway to manufacture highly active enzyme mimetics with desirable physicochemical properties.
- Subjects
HEPARIN; PEROXIDASE; PALLADIUM; AQUEOUS solutions; DETECTION limit
- Publication
Catalysis Letters, 2021, Vol 151, Issue 9, p2537
- ISSN
1011-372X
- Publication type
Article
- DOI
10.1007/s10562-021-03530-x