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- Title
Construction of recombinant Corynebacterium glutamicum for L-threonine production.
- Authors
Lv, Yangyong; Wu, Zhanhong; Han, Shuangyan; Lin, Ying; Zheng, Suiping
- Abstract
L-threonine is an essential amino acid which is widely used in feed and pharmaceutical industries. We recently engineered Corynebacterium glutamicum R102 (AHV) for improved production of L-threonine. Inactivation of genes metX and dapA encoding dihydrodipicolinate synthase and homoserine O-acetyltransferase, respectively, was firstly conducted by homologous recombination, which differed from the common random mutagenesis method. Then operon gene hom-thrB (O) and export gene thrE (E) from R102 were over-expressed alone or together to obtain a series of recombinant strains. qPCR was employed to evaluate the transcript quantification of the target genes. In flask fermentation, the newly constructed strain R102Δ metXΔ dapA (pEC-Box) was able to accumulate 3.35 g threonine/L compared with 1.80 g threonine/L of strain R102 (AHV).
- Subjects
L-Threonine dehydrogenase; AMINO acids; PHARMACEUTICAL industry; CORYNEBACTERIUM glutamicum; GENES; MUTAGENESIS
- Publication
Biotechnology & Bioprocess Engineering, 2012, Vol 17, Issue 1, p16
- ISSN
1226-8372
- Publication type
Article
- DOI
10.1007/s12257-011-0360-8