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- Title
Cell Specific Expression of Vascular Endothelial Growth Factor Receptor-2 (Flk-1/KDR) in Developing Mice Embryo and Supporting Maternal Uterine Tissue during Early Gestation (D4-D7).
- Authors
Das, Dimpimoni; Saikia, Purba J.; Gowala, Upasa; Sarma, Hirendra N.
- Abstract
Background: Vascular endothelial growth factor (VEGF) and the corresponding receptors play key role in vasculogenesis and angiogenesis processes. VEGF is one of the prime candidates in regulating embryo implantation by increasing vascular permeability. VEGF receptor-2, also called Flk-1/KDR, is one of the prime receptor which is actively involved in the execution of various functions of VEGF. However, precise role of this receptor during early gestation period is yet to be addressed. In the present study, expression of Flk-1/KDR during peri-implantation mice uterus as well as fetal-maternal tissues from day 4-day 7 (D4-D7) of gestation was investigated. Materials and Methods: In this experimental study, localization of Flk-1/KDR was investigated by immunohistochemistry and immunofluorescence techniques, in paraffin embedded tissue sections. Flk-1/KDR protein and mRNA expressions were investigated by western blotting and quantitative reverse transcription polymerase chain reaction (qRT-PCR), respectively. Effects of ovarian steroids on expression of Flk-1/KDR were also assessed by estrogen and progesterone antagonist treatment. Results: Uterine tissue on D4 showed strong expression of Flk-1/KDR in luminal and uterine glandular epithelium. On D5 and D6, differential expression of Flk-1/KDR was evidenced in certain cell types of the embryo, maternal tissues and fetal-maternal interface with varied intensity. Flk-1/KDR was specifically expressed in the ectoplacental cone (EPC) and various cells of the embryo on D7. Flk-1/KDR expression was not evidenced in the estradiol-17β (E2) and progesterone (P4) antagonist treated uterus. Western blotting result revealed presence of Flk-1/KDR protein in all the gestation days, except antagonist treated uterus. qRT-PCR analysis showed significant increase of Flk-1/KDR mRNA transcript on D6 and D7. Conclusion: Spatial-temporal expression of Flk-1/KDR during peri-implantation period in mice uterus especially in the feto-maternal interface was observed. This spatio-temporal specificity as well as increased expression of Flk-1/KDR could be one of the determinants for establishment of fetal-maternal cross talk during the critical period of development.
- Subjects
PROGESTERONE antagonists; STEROID drugs; BIOLOGICAL models; EXPERIMENTAL design; REVERSE transcriptase polymerase chain reaction; ANIMAL experimentation; IMMUNOHISTOCHEMISTRY; WESTERN immunoblotting; ESTROGEN antagonists; GENE expression; UTERUS; FLUORESCENT antibody technique; MESSENGER RNA; VASCULAR endothelial growth factors; POLYMERASE chain reaction; TRANSCRIPTION factors; MICE
- Publication
International Journal of Fertility & Sterility, 2021, Vol 15, Issue 2, p148
- ISSN
2008-076X
- Publication type
Article
- DOI
10.22074/IJFS.2021.134530